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. Author manuscript; available in PMC: 2011 May 10.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2009 Oct 1;75(2):534–542. doi: 10.1016/j.ijrobp.2009.06.034

Figure 2. Radiation induced NF-κB in colorectal cancer cells.

Figure 2

Dose dependent increase in NF-κB activity was observed in HCT116 (a), HT29 (c), and SW620 (e) cells. Colon cancer cells (2 × 105/mL) were exposed to graded doses of radiation and harvested 3 h after irradiation. Nuclear extracts were probed for NF-κB activation by EMSA. The time course of radiation-induced NF-κB activation after 10 Gy irradiation in HCT116 (b), HT29 (d), and SW620 (f). Cells (2 × 105 /mL) were exposed to radiation, harvested post-radiation at indicated time points, and probed for NF-κB. Activation of NF-κB was expressed as fold increase of DNA-bound protein over untreated controls (basal levels). The band labeled NF-κB represents the p65:p50 heterodimer, the predominant functionally active NF-κB dimer whereas the lower band is a non-specific band formed by the inactive NF-κB homodimer (p50:p50)