Figure 4.

Effect of NAC or L-NAC pretreatment on mitochondrial membrane potential following PQ challenge. Cells pretreated for 4 h with control media or 5.0 mM NAC- (N) or L-NAC-containing media (L) were challenged with 0, 0.25, or 1.0 mM PQ for 4 h. Cells were stained for 30 min posttreatment with the cell permeable JC-1 fluorescent dye. Bars represent mean ± S.E.M. of 3 independent experiments. *denotes significant difference relative to cells with no pretreatment (P < .05).