Complementation of the late-flowering phenotype of axe1-5 by overexpressing 35S:HDA6-FLAG. A, axe1-5 and the complementation lines (axe-C1 and axe-C2) grown under LD conditions compared with the Col wild type. B, Rosette leaf numbers at bolting of Col, axe1-5, axe-C1, and axe-C2 plants grown under LD and SD conditions. At least 20 plants were scored for each line. Error bars indicate sd. * Under SD conditions, most axe1-5 plants did not flower 120 d after germination, and the rosette leaf number was counted at 120 d. C, qRT-PCR analysis of the expression of FLC, MAF4, and MAF5 in Col, axe1-5, axe-C1, and axe-C2 plants grown under LD conditions for 20 d. [See online article for color version of this figure.]