HDA6 interacted with FLD in yeast two-hybrid assays. A, Different regions of HDA6 fused with the Gal4 binding domain (BD) and the full-length FLD fused with the Gal4 activation domain (AD) were cotransformed into the yeast strain AH109. B, Different regions of FLD fused with AD and the C-terminal fragment of HDA6 fused with BD were cotransformed into the yeast strain AH109. The transformants were plated onto plates with SD/-Leu-Trp-His/3-amino-1,2,4-triazole/5-bromo-4-chloro-3-indolyl-α-d-galactopyranoside for 3 d. aa, Amino acids. [See online article for color version of this figure.]