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. 2011 Mar 22;156(1):228–239. doi: 10.1104/pp.111.175042

Figure 1.

Figure 1.

Both BBX22 protein and transcript are transiently accumulated. A, A BBX22-specific antibody was generated and used to determine the steady-state protein level by immunoblot analysis. Proteins were isolated from 4-d-old etiolated (dark; D) and 12-h light-treated (L) ecotype Columbia (Col) or bbx22 seedlings. Endogenous α-tubulin was a loading control (TUB). B and C, BBX22 protein (B) and mRNA (C) are transiently accumulated during photomorphogenesis. Immunoblotting was used to detect the accumulation of BBX22 in etiolated seedlings and seedlings illuminated with light for 3 to 24 h. Real-time quantitative RT-PCR was used to monitor the expression of endogenous BBX22 in C. The expression of UBQ10 in each sample was used as an internal control. The BBX22 expression in etiolated seedlings was set to 1. The expression of BBX22 is presented as the amount of increase at each time point relative to that in etiolated seedlings and represented as “ratio.” The means and sd were calculated from three replicates and plotted.