Figure 3.
BBX22 is a short-lived protein degraded by the 26S proteasome. The degradation kinetics of BBX22 protein were determined as a percentage of full-length BBX22 remaining relative to BBX22 at 8 h of light treatment in the presence of cycloheximide. Half-life (t1/2) was calculated by regression analysis. The half-life of BBX22 is 60 min in the light (white circles, solid lines) and 20 min in the dark (black circles, solid lines). The degradation of BBX22 was blocked by treatment with MG132 under both light (white circles, dashed lines) and dark (black circles, dashed lines) conditions. n = 3.