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. 2011 Mar 24;156(1):165–172. doi: 10.1104/pp.111.172734

Figure 1.

Figure 1.

The scaffolding A subunits of PP2A are required for light activation of NR. A, Relative transcript levels for the PP2AA subunit in tamoxifen-treated wild-type and amiRNA-1 plants, and mock-treated plants. Expression levels were tested with RT-PCR using SYBR green with plant samples also used for testing NR activation. se for the RT-PCR assay is given, n = 3. B, Activity state of NR following a dark-to-light transition of wild-type (dots) and amiRNA-1 (squares) plants treated with tamoxifen (black symbols), and mock-treated plants (white symbols). Plants were grown in 12-h light/12-h darkness for 3 weeks. Two hours into the photoperiod, when NR activity was at a high level, plants were transferred to darkness and samples harvested after 1 h of darkness (time zero), then after 20 and 40 min of white light (150 μmol m−2 s−1). Data are means of three samples, se is given.