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. 2011 Mar 22;156(1):144–164. doi: 10.1104/pp.111.172502

Figure 5.

Figure 5.

In Arabidopsis roots, IAA and ACC differentially induce gene expression, which predicts metabolite accumulation kinetics. A and B, Kinetic analysis of CHS, F3′H, and FLS expression via qRT-PCR after treatment with 1 μm IAA (A) and ACC (B). Abundance of the mRNA from three genes relative to actin is represented as fold increase over untreated controls. C and D, Q-DPBA- and K-DPBA-specific fluorescence in primary roots stained with DPBA was monitored over time in response to IAA (C) and ACC (D). E and F, Kinetic analysis of IAA-induced (E) and ACC-induced (F) changes in CHS, MYB12, TTG1, and PAP1 expression determined from RNA samples used in A and B. Average and se of normalized gene expression from three pools of individuals are shown in A, B, E, and F; average and se of DPBA fluorescence from three independent trials containing eight individual roots each are shown in C and D.