Table I. Ratios of target gene transcript to actin in control, IAA-, and ACC-treated wild-type samples as determined by qRT-PCR.

Roots were transferred to control medium or medium containing 1 μm ACC or IAA for 4 h. RNA was harvested from roots and amplified by qRT-PCR with gene-specific primers. The average ± se of three biological replicates is reported. Fold change, shown in parentheses, was determined by dividing the target actin ratios of the treated sample by the control ratio. ND, Not detected.

Gene	Transcript Abundance Relative to Actin
Control	IAA	ACC
CHS	0.085 ± 0.008	0.28 ± 0.06 (3.3)	0.25 ± 0.05 (3.0)
CHI	0.048 ± 0.006	0.19 ± 0.03 (3.9)	0.16 ± 0.02 (3.3)
F3H	0.011 ± 0.002	0.011 ± 0.003 (1.0)	0.012 ± 0.002 (1.1)
FLS	0.023 ± 0.004	0.10 ± 0.02 (4.5)	0.065 ± 0.004 (2.8)
F3′H	0.0059 ± 0.0006	0.022 ± 0.003 (3.7)	0.0089 ± 0.0005 (1.5)
DFR	ND	ND	ND
MYB12	0.022 ± 0.002	0.31 ± 0.05 (14.2)	0.15 ± 0.04 (6.9)
TTG1	0.0082 ± 0.0004	0.033 ± 0.003 (4.1)	0.027 ± 0.004 (3.3)
PAP1	0.015 ± 0.004	0.058 ± 0.004 (3.9)	0.047 ± 0.006 (3.1)