Functional analysis of selected TFs during Arabidopsis embryo development. Nomarski images of embryo-defective phenotypes observed with translational fusions of TFs with En repressor domain under the control of 35S promoter (“Materials and Methods”). Putative zinc-finger TFs: At3G24050 (A); At3G51080 (B); At5G66320 (C); At3G54810 (D); At4G32890 (E); HD-ZIP At4G37790 (F); BELL1 LIKE homeodomain, A4G34610 (G); WOX6, At2G28610 (H); TCP16, At3G45150 (I); GRAS family SCL7, At3G50650 (J); and MYB, At3G55730 (K). Embryo phenotypes (arrows) observed include arrest at two-cell stage (E), quadrant (G), and octant (I); defective hypophyseal cell division (J); abnormal divisions in the basal region of the embryo at later stages of development (A, B, E, F, H, and K); abnormal divisions in the upper suspensor cells (B and C); and abnormal endosperm cell division (D and I). Details of observed phenotypes summarized in Supplemental Table S8d. Bar = 0.01 mm.