Skip to main content
. 2011 Mar 8;156(1):404–416. doi: 10.1104/pp.110.167510

Figure 3.

Figure 3.

SI stimulates increases in ROS in incompatible pollen tubes. A, Normally growing pollen tube labeled with CM-H2DCF-DA to monitor ROS by formation of fluorescent CM-DCF 1 min after SI induction. B, The same pollen tube 4 min after SI induction. C, CM-DCF fluorescence in a growing pollen tube before SI induction. D to I, The same pollen tube 2 min after SI induction (D) and 7 min (E), 9 min (F), 12 min (G), 14 min (H), and 20 min (I) after SI induction. J, Bright-field image at the end of the series. Bars = 10 μm. K, Representative examples of quantitation of CM-DCF in untreated pollen tube (white symbols) and after SI challenge induced at time zero (arrow; black symbols). Measurements are of relative fluorescence units (RFU) measured in individual pollen tubes.