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. 2011 Mar 8;156(1):404–416. doi: 10.1104/pp.110.167510

Figure 4.

Figure 4.

SI stimulates increases in NO in incompatible pollen tubes. Pollen tubes were labeled with DAF-FM-DA. A to F, Typical DAF-FM-T signal in a pollen tube imaged after SI induction. Images are before SI induction (A) and 23 min (B), 27 min (C), 31 min (D), 53 min (E), and 63 min (F) after SI. G to J, Typical DAF-FM-T signal in an untreated pollen tube imaged for a similar time series. Images are at 0 min (G), 24 min (H), 34 min (I), and 45 min (J). Bar = 10 μm. K, Representative examples of quantitation of DAF-FM-T fluorescence in an untreated pollen tube (white symbols) and after SI challenge (arrow; black symbols). Measurements are of relative fluorescence units (RFU) measured in individual pollen tubes.