Effects of ethylene-responsive mutations and inhibitors of ethylene biosynthesis or response on the hypoxic induction of AtERF73/HRE1. A, Quantitative RT-PCR analysis of AtERF73/HRE1expression in ein2-5 and etr1-1 mutants under hypoxic conditions. Total RNAs were isolated from roots of Col-0, ein2-5, and etr1-1 after hypoxia treatment for 6 h. Levels of AtERF73/HRE1 gene expression were determined. B, Expression levels of AtERF73/HRE1 and ADH in Arabidopsis seedlings after 6 h of hypoxia in the presence of 10 μm AVG and 10 μm AgNO3 were determined by quantitative RT-PCR, and the relative amounts of transcripts were calculated and normalized with β-ATP mRNA. Values represent means ± sd from three biologically independent experiments. * P < 0.05 versus the wild type (one-way ANOVA with Dunnett’s t test).