Figure 6.
Heterologous MUM2 expression in mutant lines. A, Seed obtained from a class I primary mum2;LUHpro::MUM2 transformant showing restored mucilage extrusion following ruthenium red staining. B to D, Seeds obtained from class I (B), class II (C), and class III (D) primary luh;LUHpro::MUM2 transformants. Bars = 250 μm. E, Histogram showing mol % of sugar linkages associated with the RG-I backbone and side chains present in the alkali-soluble fraction of mucilage extracted from wild-type (wt) and transgenic mutant lines. The complete data set is presented in Supplemental Table S1.