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. 2011 Mar 14;156(1):46–60. doi: 10.1104/pp.111.172692

Table I. Sugar linkage composition of extracted mucilage from wild-type, luh, and mum2 seeds.

Soluble polysaccharides from intact seeds were extracted sequentially with acid (HCl soluble) and alkali (NaOH soluble). Samples were neutralized, and following methylation, the partially methylated alditol acetates were quantified by GC-MS. Results are given as mol % ± se calculated from two independent experiments (wild type and luh). ND, Not detected; tr, trace (less than 0.5 mol %).

Sugar and Linkage HCl Soluble NaOH Soluble
Wild Type luh mum2 Wild Type luh mum2
Rha
t-Rhap 0.9 ± 0.5 tr 0.5 1.0 ± 0.0 0.9 ± 0.1 tr
 2-Rhap 44.1 ± 4.6 28.3 ± 1.5 30.9 28.5 ± 3.9 22.8 ± 1.2 23.0
 2,4-Rhap 1.0 ± 0.5 7.0 ± 3.2 5.7 2.4 ± 0.2 9.2 ± 0.7 5.8
 Total 46 35.3 37.1 31.9 32.9 28.8
Ara
t-Araf 2.4 ± 0.0 5.0 ± 2.0 3.0 5.9 ± 2.1 4.0 ± 1.9 2.0
 2-Araf 0.5 ± 0.2 0.8 ± 0.1 0.6 2.9 ± 2.5 2.2 ± 2.5 2.1
 3-Araf 0.5 ± 0.1 0.6 ± 0.1 0.5 1.7 ± 0.8 1.0 ± 0.9 1.1
 5-Araf 0.8 ± 0.2 2.0 ± 1.8 1.4 2.0 ± 0.8 1.4 ± 1.4 1.0
 2,5-Araf 0.5 ± 0.1 0.6 ± 0.1 0.8 1.1 ± 1.5 0.6 ± 0.9 ND
 3,5-Araf tr tr ND tr tr ND
 Total 4.7 9 6.3 13.6 9.2 6.2
Xyl
t-Xylp tr 0.7 ± 0.6 0.5 3.1 ± 1.4 2.5 ± 1.7 0.5
 4-Xylp tr 1.6 ± 2.2 1.0 5.3 ± 4.2 49.2.9 5.4
 Total 0 2.3 1.5 8.4 7.4 5.9
Man
 4-Manp 0.5 ± 0.7 1.7 ± 2.4 1.2 1.8 ± 2.5 1.5 ± 2.1 2.1
Gal
t-Galp 0.8 ± 0.3 8.1 ± 0.7 8.4 1.7 ± 0.3 8.9 ± 0.1 8.0
 3-Galp tr 1.0 ± 0.7 0.8 1.3 ± 0.0 1.0 ± 0.2 4.0
 4-Galp 0.6 ± 0.3 0.9 ± 0.8 tr 1.1 ± 1.1 1.1 ± 1.1 ND
 6-Galp tr tr ND 0.5 ± 0.8 tr ND
 3,6-Galp tr 0.6 ± 0.9 ND 0.5 ± 0.7 0.5 ± 0.7 ND
 Total 1.4 10.6 9.2 5.1 11.5 12
Glc
t-Glcp tr tr tr 1.6 ± 1.8 1.2 ± 1.1 2.1
 2-Glcp tr tr ND tr 1.2 ± 0.7 1.5
 4-Glcp 0.9 ± 0.3 1.9 ± 1.5 7.9 4.5 ± 1.4 2.5 ± 0.7 7.9
 3,4-Glcp tr tr ND 0.7 ± 1.1 0.7 ± 1.0 ND
 Total 0.9 1.9 7.9 6.8 5.6 11.5
GalUA
t-GalAp 1.0 ± 0.3 0.9 ± 0.7 1.5 1.0 ± 0.1 1.2 ± 0.4 tr
 4-GalAp 42.0 ± 1.3 35.1 ± 2.4 33.9 28.6 ± 0.7 30.1 ± 0.1 32.5
 Total 43 36 35.4 29.6 31.3 32.5
GlcA
t-GlcAp tr 0.7 ± 0.5 0.7 1.1 ± 1.6 0.5 ± 0.1 tr