Table I. Sugar linkage composition of extracted mucilage from wild-type, luh, and mum2 seeds.
Sugar and Linkage | HCl Soluble | NaOH Soluble | ||||
Wild Type | luh | mum2 | Wild Type | luh | mum2 | |
Rha | ||||||
t-Rhap | 0.9 ± 0.5 | tr | 0.5 | 1.0 ± 0.0 | 0.9 ± 0.1 | tr |
2-Rhap | 44.1 ± 4.6 | 28.3 ± 1.5 | 30.9 | 28.5 ± 3.9 | 22.8 ± 1.2 | 23.0 |
2,4-Rhap | 1.0 ± 0.5 | 7.0 ± 3.2 | 5.7 | 2.4 ± 0.2 | 9.2 ± 0.7 | 5.8 |
Total | 46 | 35.3 | 37.1 | 31.9 | 32.9 | 28.8 |
Ara | ||||||
t-Araf | 2.4 ± 0.0 | 5.0 ± 2.0 | 3.0 | 5.9 ± 2.1 | 4.0 ± 1.9 | 2.0 |
2-Araf | 0.5 ± 0.2 | 0.8 ± 0.1 | 0.6 | 2.9 ± 2.5 | 2.2 ± 2.5 | 2.1 |
3-Araf | 0.5 ± 0.1 | 0.6 ± 0.1 | 0.5 | 1.7 ± 0.8 | 1.0 ± 0.9 | 1.1 |
5-Araf | 0.8 ± 0.2 | 2.0 ± 1.8 | 1.4 | 2.0 ± 0.8 | 1.4 ± 1.4 | 1.0 |
2,5-Araf | 0.5 ± 0.1 | 0.6 ± 0.1 | 0.8 | 1.1 ± 1.5 | 0.6 ± 0.9 | ND |
3,5-Araf | tr | tr | ND | tr | tr | ND |
Total | 4.7 | 9 | 6.3 | 13.6 | 9.2 | 6.2 |
Xyl | ||||||
t-Xylp | tr | 0.7 ± 0.6 | 0.5 | 3.1 ± 1.4 | 2.5 ± 1.7 | 0.5 |
4-Xylp | tr | 1.6 ± 2.2 | 1.0 | 5.3 ± 4.2 | 49.2.9 | 5.4 |
Total | 0 | 2.3 | 1.5 | 8.4 | 7.4 | 5.9 |
Man | ||||||
4-Manp | 0.5 ± 0.7 | 1.7 ± 2.4 | 1.2 | 1.8 ± 2.5 | 1.5 ± 2.1 | 2.1 |
Gal | ||||||
t-Galp | 0.8 ± 0.3 | 8.1 ± 0.7 | 8.4 | 1.7 ± 0.3 | 8.9 ± 0.1 | 8.0 |
3-Galp | tr | 1.0 ± 0.7 | 0.8 | 1.3 ± 0.0 | 1.0 ± 0.2 | 4.0 |
4-Galp | 0.6 ± 0.3 | 0.9 ± 0.8 | tr | 1.1 ± 1.1 | 1.1 ± 1.1 | ND |
6-Galp | tr | tr | ND | 0.5 ± 0.8 | tr | ND |
3,6-Galp | tr | 0.6 ± 0.9 | ND | 0.5 ± 0.7 | 0.5 ± 0.7 | ND |
Total | 1.4 | 10.6 | 9.2 | 5.1 | 11.5 | 12 |
Glc | ||||||
t-Glcp | tr | tr | tr | 1.6 ± 1.8 | 1.2 ± 1.1 | 2.1 |
2-Glcp | tr | tr | ND | tr | 1.2 ± 0.7 | 1.5 |
4-Glcp | 0.9 ± 0.3 | 1.9 ± 1.5 | 7.9 | 4.5 ± 1.4 | 2.5 ± 0.7 | 7.9 |
3,4-Glcp | tr | tr | ND | 0.7 ± 1.1 | 0.7 ± 1.0 | ND |
Total | 0.9 | 1.9 | 7.9 | 6.8 | 5.6 | 11.5 |
GalUA | ||||||
t-GalAp | 1.0 ± 0.3 | 0.9 ± 0.7 | 1.5 | 1.0 ± 0.1 | 1.2 ± 0.4 | tr |
4-GalAp | 42.0 ± 1.3 | 35.1 ± 2.4 | 33.9 | 28.6 ± 0.7 | 30.1 ± 0.1 | 32.5 |
Total | 43 | 36 | 35.4 | 29.6 | 31.3 | 32.5 |
GlcA | ||||||
t-GlcAp | tr | 0.7 ± 0.5 | 0.7 | 1.1 ± 1.6 | 0.5 ± 0.1 | tr |