Figure 1.

Schematic overview of the T-DNA region of the expression constructs generated in this study. ^wt-Ph2G12sc_SEC, ^wt-Ph2G12sc_KDEL, ^wt-PhHA78sc_SEC, and ^wt-PhHA78sc_KDEL were cloned into the vector pPhasGW (F. Morandini, B. Van Droogenbroeck, and A. Depicker, unpublished data) for transformation into wild-type and TKO plants. ^wt-35S2G12sc_SEC and ^wt-35SHA78sc_SEC were additionally cloned into the binary expression vector pPT2 (Strasser et al., 2005). LB, Left border; 3′ocs, 3′ end of the octopine synthase gene; nptII, neomycin phosphotransferase II; Pnos, nopaline synthase promoter; Pphas, β-phaseolin promoter (1–1,470; GenBank accession no. J01263); attB1, attB2, attP1, attP2, attL1, attL2, attR1, and attR2, recombination sites for Gateway cloning (Invitrogen, 2003); CmR-ccdB, Gateway positive/negative selection cassette; 3′arc5-I, approximately 4,000 bp of 3′ flanking region of the arceline 5I gene (part of GenBank accession no. Z50202); RB, right border; 2S2, signal peptide of the Arabidopsis 2S2 seed storage protein (Krebbers et al., 1988); KDEL, ER retrieval motif; Tnos, nopaline synthase terminator; P35S, cauliflower mosaic virus 35S promoter; g7T, 200 bp of transcript 7 3′ region (bp 398–598, Dhaese et al., 1983).