Figure 2.

Immunodetection of β-phaseolin-driven scFv-Fcs extracted from seeds. One microliter of crude seed extracts (corresponding to 10 μg of seeds) was separated by SDS-PAGE, blotted on a nitrocellulose membrane, and the scFv-Fcs were detected with goat anti-human IgG (H+L) HRP conjugate (Promega; W403B) and a chemiluminescent substrate (SuperSignal West Pico Chemiluminescent Substrate; Pierce). After 3 s of film exposure, all constructs could be visualized. Arrowheads indicate degradation fragments, and bars indicate the height of marker bands (in kD).