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. 2011 Feb 16;155(4):2036–2048. doi: 10.1104/pp.110.171330

Figure 4.

Figure 4.

N-Glycosylation patterns of different seed- and leaf-produced scFv-Fcs. N-Glycan analysis was carried out by LS-ESI-MS of tryptic peptides. Due to incomplete tryptic digest, each glycan structure is represented by two glycopeptides assigned -1 and -2. All 2G12 constructs as well as wt-PhHA78scKDEL carry exclusively oligomannosidic N-glycans. wt-PhHA78scSEC carries xylosylated and fucosylated complex-type N-glycans as well as oligomannosidic structures, while TKO-PhHA78scSEC carries nonxylosylated and nonfucosylated complex-type N-glycans as well as oligomannosidic structures. wt-35SHA78scSEC extracted from leaves carries xylosylated and fucosylated complex-type N-glycans. The depicted glycoforms show one of the possible isoforms. For N-glycan nomenclature, see www.proglycan.com [See online article for color version of this figure.]