Figure 8.
Subcellular localization of wt-Ph2G12scSEC in developing Arabidopsis seeds by IEM. A, Gold label was mainly found in ERVs. The nuclear envelope was partially swollen and labeled in a density similar to the ERVs. B, The ERVs were partially ribosome studded (arrowheads), indicating ER origin. Gold label was not found in the Golgi apparatus. C, ERVs were abundant structures. Some ERVs are indicated by arrows. D, The PSV was not labeled, but a Golgi stack was slightly labeled. E, The extracellular space was devoid of labeling. cl, Chloroplast; CW, cell wall; EC, extracellular space; G, Golgi apparatus; N, nucleus; NE, nuclear envelope; OB, oil body; SNE, swollen nuclear envelope. Bars = 200 nm (A, B, D, and E) and 1,000 nm (C).