Table I. Overview of scFv-Fc-expressing lines.
Name | scFv-Fc Based on mAb | Targeting Signals | Expression Vector | Promoter | Genetic Background |
wt-Ph2G12scSEC | 2G12 | For secretion | pPhasGW | β-Phaseolin | Wild type |
wt-Ph2G12scKDEL | 2G12 | For ER retention | pPhasGW | β-Phaseolin | Wild type |
TKO-Ph2G12scSEC | 2G12 | For secretion | pPhasGW | β-Phaseolin | TKO |
wt-35S2G12scSEC | 2G12 | For secretion | pPT2 | 35S | Wild type |
wt-PhHA78scSEC | HA78 | For secretion | pPhasGW | β-Phaseolin | Wild type |
wt-PhHA78scKDEL | HA78 | For ER retention | pPhasGW | β-Phaseolin | Wild type |
TKO-PhHA78scSEC | HA78 | For secretion | pPhasGW | β-Phaseolin | TKO |
wt-35SHA78scSEC | HA78 | For secretion | pPT2 | 35S | Wild type |