Table I. Proteins with increased levels in response to zinc in the wild type.

Microsomal proteins from the wild type grown on MS or 300-Zn medium were labeled with iTRAQ-114 and -115 reagent, respectively, and those from the det3-1 mutant grown on MS or 300-Zn medium were labeled with iTRAQ-116 and -117 reagent, respectively. Proteins that increased more than 2.0-fold were selected and ordered based on fold change values in wild type 300-Zn/wild type MS. Data are means ± sd of three independent experiments. * P < 0.05, ** P < 0.001, significantly different from the wild type or the det3-1 mutant on MS medium.

Arabidopsis Genome Initiative Code	Protein	Peptidea	Coverageb	Ratioc (Wild Type 300-Zn/Wild Type MS)	Ratioc (det3-1 MS/Wild Type MS)	Ratioc (det3-1 300-Zn/det3-1 MS)
AT4G10480	Nascent polypeptide-associated  complex α-chain protein, putative/α-NAC, putative	3.3	23.9	12.111 ± 3.947*	0.689 ± 0.243	6.328 ± 3.262*
AT3G12390	Nascent polypeptide-associated complex α-chain protein, putative/α-NAC, putative	3.0	20.5	12.051 ± 3.382*	0.957 ± 0.205	5.102 ± 3.486
AT3G49470	NACA2 (nascent polypeptide-associated complex subunit α-like protein 2)	1.0	6.0	11.726 ± 7.023	0.720 ± 0.010**	5.409 ± 1.887*
AT4G19690	IRT1 (iron-regulated transporter 1); cadmium, iron, manganese, and zinc transmembrane transporter	3.7	19.3	10.742 ± 5.370*	0.664 ± 0.130*	15.066 ± 8.097*
AT1G01580	FRO2 (ferric reduction oxidase 2);ferric-chelate reductase	12.0	18.0	8.449 ± 1.787**	0.738 ± 0.085*	5.764 ± 0.762**
AT1G09560	GLP5 (germin-like protein 5); manganese ion binding/metal ion binding/nutrient reservoir	4.0	15.7	5.026 ± 1.528*	0.723 ± 0.133*	3.940 ± 1.076*
AT3G58810	MTP3/MTPA2; efflux transmembrane transporter/zinc ion transmembrane transporter	4.3	8.7	4.708 ± 0.207**	0.649 ± 0.052**	4.658 ± 1.791*
AT1G17880	Nascent polypeptide-associated complex domain-containing protein/BTF3b-like transcription factor, putative	6.0	37.6	4.375 ± 1.211*	0.875 ± 0.037*	2.650 ± 1.013
AT1G20200	EMB2719 (embryo defective 2719)	5.0	12.6	3.787 ± 1.055*	0.893 ± 0.127	2.739 ± 2.509
AT1G78000	SULTR1;2 (sulfate transporter 1;2); sulfate transmembrane transporter	5.7	9.5	3.736 ± 0.678**	0.718 ± 0.048**	2.357 ± 0.383*
AT1G73230	Nascent polypeptide-associated complex domain-containing protein	7.0	37.8	3.652 ± 0.649**	0.862 ± 0.014**	2.483 ± 0.874*
AT4G16190	Cys proteinase, putative	3.0	12.2	3.054 ± 0.374**	0.614 ± 0.082**	4.550 ± 0.083**
AT3G51800	ATG2 (G2p-related protein);metalloexopeptidase	8.3	22.8	2.820 ± 0.465*	0.988 ± 0.112	2.278 ± 0.630*
AT1G78850	Curculin-like (Man-binding) lectin family protein	5.3	15.1	2.737 ± 0.536*	1.014 ± 0.132	2.104 ± 0.774*
AT4G39090	RD19 (responsive to dehydration 19); Cys-type peptidase	5.3	17.0	2.719 ± 0.270**	0.601 ± 0.076**	4.205 ± 0.352**
AT3G53890	40S ribosomal protein S21 (RPS21B)	1.7	17.5	2.661 ± 0.796*	0.994 ± 0.118	1.690 ± 0.565
AT3G10090	40S ribosomal protein S28 (RPS28A)	3.0	39.1	2.531 ± 0.236**	0.728 ± 0.010**	2.015 ± 0.454*
AT5G64140	RPS28 (ribosomal protein S28); structural constituent of ribosome	3.0	39.1	2.521 ± 0.171**	0.711 ± 0.025**	2.038 ± 0.434*
AT2G07727	Cytochrome b (MTCYB, COB, CYTB)	1.0	3.3	2.492 ± 0.380*	1.041 ± 0.019*	1.093 ± 0.142
AT3G30390	Amino acid transporter family protein	2.7	6.1	2.359 ± 0.100**	0.789 ± 0.108*	1.978 ± 0.393*
AT2G27730	Identical to uncharacterized mitochondrial protein; similar to GB:CAN62125.1 and GB:CAO48731.1	1.7	19.8	2.351 ± 0.566*	0.984 ± 0.056	1.159 ± 0.235
AT4G32470	Ubiquinol-cytochrome c reductase complex 14-kD protein, putative	2.3	21.3	2.279 ± 0.547*	0.897 ± 0.087	1.021 ± 0.163
AT5G19820	EMB2734 (embryo defective 2734); lyase	2.7	3.0	2.247 ± 0.365*	0.389 ± 0.044**	3.975 ± 1.456*
AT1G48630	Guanine nucleotide-binding family protein/activated protein kinase C receptor, putative/RACK, putative	7.3	20.4	2.199 ± 0.401*	0.357 ± 0.030**	3.774 ± 1.809
AT1G18080	ATARCA (Arabidopsis thaliana homolog of tobacco ArcA); nucleotide binding	14.0	38.3	2.123 ± 0.408*	0.372 ± 0.023**	3.714 ± 1.685
AT1G51980	Mitochondrial processing peptidase α-subunit, putative	4.3	12.0	2.081 ± 0.383*	0.878 ± 0.186	1.141 ± 0.082*
AT1G26630	FBR12 (fumonisin B1-resistant 12); translation initiation factor	6.3	38.8	2.076 ± 0.145**	0.808 ± 0.025**	1.464 ± 0.226*

^aPeptide indicates average number of assigned peptides.

^bCoverage indicates average percentage of assigned peptides to the predicted protein.

^cThe values were calculated as the ratio of 115 (wild type 300-Zn) to 114 (wild type MS) label, 116 (det3-1 MS) to 114 (wild type MS) label, or 117 (det3-1 300-Zn) to 116 (det3-1 MS) label.