Table IV. Effect of excess zinc on protein levels of V-ATPase subunits and V-PPase.
Microsomal proteins from the wild type grown on MS or 300-Zn medium were labeled with iTRAQ-114 and -115 reagent, respectively, and those from the det3-1 mutant grown on MS or 300-Zn medium were labeled with iTRAQ-116 and -117 reagent, respectively. Subunits of V-ATPase complex and V-PPase were selected and alphabetically ordered. Boldface entries indicate the V-ATPase subunits that were significantly decreased in both the wild type and the det3-1 mutant. Data are means ± sd of three independent experiments. N.D. indicates that a peptide and/or iTRAQ reporter ions were not detected. * P < 0.05, ** P < 0.001, significantly different from the wild type or the det3-1 mutant on MS medium.
| Arabidopsis Genome Initiative Code | Protein | Peptidea | Coverageb | Ratioc (Wild Type 300-Zn/Wild Type MS) | Ratioc (det3-1 MS/Wild Type MS) | Ratioc (det3-1 300-Zn/det3-1 MS) |
| AT1G78900 | VHA A | 25.0 | 47.2 | 0.809 ± 0.076* | 0.570 ± 0.036** | 0.758 ± 0.148 |
| AT1G76030 | VHA B1 | N.D. | N.D. | N.D. | ||
| AT4G38510 | VHA B2 | N.D. | N.D. | N.D. | ||
| AT1G20260 | VHA B3 | N.D. | N.D. | N.D. | ||
| AT1G12840 | VHA C | 7.3 | 21.3 | 0.795 ± 0.196 | 0.397 ± 0.054** | 0.791 ± 0.125* |
| AT3G58730 | VHA D | 8.3 | 30.4 | 0.893 ± 0.097 | 0.604 ± 0.048** | 0.832 ± 0.249 |
| AT4G11150 | VHA E1 | 12.7 | 48.0 | 0.832 ± 0.044* | 0.568 ± 0.045** | 0.766 ± 0.143 |
| AT3G08560 | VHA E2 | N.D. | N.D. | N.D. | ||
| AT1G64200 | VHA E3 | N.D. | N.D. | N.D. | ||
| AT4G02620 | VHA F | N.D. | N.D. | N.D. | ||
| AT3G01390 | VHA G1 | 5.3 | 59.1 | 0.983 ± 0.076 | 0.589 ± 0.060** | 0.847 ± 0.019** |
| AT4G23710 | VHA G2 | N.D. | N.D. | N.D. | ||
| AT4G25950 | VHA G3 | N.D. | N.D. | N.D. | ||
| AT3G42050 | VHA H | 8.3 | 25.6 | 0.835 ± 0.324 | 0.696 ± 0.065** | 0.859 ± 0.103 |
| AT2G28520 | VHA a1 | N.D. | N.D. | N.D. | ||
| AT2G21410 | VHA a2 | N.D. | N.D. | N.D. | ||
| AT4G39080 | VHA a3 | 7.0 | 9.5 | 1.074 ± 0.144 | 0.739 ± 0.063** | 1.044 ± 0.274 |
| AT4G34720 | VHA c1 | N.D. | N.D. | N.D. | ||
| AT1G19910 | VHA c2 | 2.0 | 15.2 | 1.274 ± 0.119* | 0.835 ± 0.046* | 1.384 ± 0.558 |
| AT4G39080 | VHA c3 | N.D. | N.D. | N.D. | ||
| AT1G75630 | VHA c4 | N.D. | N.D. | N.D. | ||
| AT2G16510 | VHA c5 | N.D. | N.D. | N.D. | ||
| AT4G32530 | VHA c”1 | N.D. | N.D. | N.D. | ||
| AT2G25610 | VHA c”2 | N.D. | N.D. | N.D. | ||
| AT3G28710 | VHA d1 | N.D. | N.D. | N.D. | ||
| AT3G28715 | VHA d2 | N.D. | N.D. | N.D. | ||
| AT5G55290 | VHA e1 | N.D. | N.D. | N.D. | ||
| AT4G26710 | VHA e2 | N.D. | N.D. | N.D. | ||
| AT1G15690 | AVP1 | 12.0 | 14.3 | 1.450 ± 0.172* | 1.331 ± 0.113* | 1.062 ± 0.256 |
Peptide indicates average number of assigned peptides.
Coverage indicates average percentage of assigned peptides to the predicted protein.
The values were calculated as the ratio of 115 (wild type 300-Zn) to 114 (wild type MS) label, 116 (det3-1 MS) to 114 (wild type MS) label, or 117 (det3-1 300-Zn) to 116 (det3-1 MS) label.