Figure 6.
Endo-β-1,4-mannanase enzyme activity during CAP weakening. A, Time course of endo-β-1,4-mannanase enzyme activity in separate tissues. B, Endo-β-1,4-mannanase enzyme activity in the RAD, CAP, NME, and cotyledons at three time points. C, The pattern of LesaMAN7 transcript expression in different tissues during CAP weakening. Note that in B and C, values at 36 h are measured separately in tissues from seeds with and without CAP rupture. GenBank accession numbers for LesaMAN7 and LesaACT7 are HQ436349 and HQ436350, respectively. RT, Reverse transcription. [See online article for color version of this figure.]