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BBL down-regulation in tobacco plants. Tobacco plants of the wild type (W) and six independent BBL RNAi lines (KP1–KP6) were grown for 1 month after germination and analyzed for gene expression and alkaloids. A, Immunoblot analysis of BBL and PEPC (control) proteins in the root. B and C, Levels of nicotine, DMN, and nornicotine in the fourth newest leaf (B) and the root (C). Data indicate mean values ± sd from three replicates. ND, Not detected.
