Figure 7.

BBL down-regulation in cultured tobacco cells. Cultured BY-2 cells of the wild type (WT), two vector-transformed control cell lines (VC1 and VC2), and two BBL RNAi lines (KB1 and KB2) were treated with 50 μm MeJA for 48h (+), and their protein levels and alkaloid contents were analyzed. Wild-type BY-2 cells were also cultured in the absence of MeJA (−). A, Immunoblot analysis of BBL and PEPC (control) proteins. B, Anatabine content. C, Levels of nicotine, anabasine, and anatalline. Note that the scale is different from that in B. Data indicate mean values ± sd from three replicates. ND, Not detected.