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. 2011 Feb 18;155(4):1806–1816. doi: 10.1104/pp.110.171082

Table I. Kinetic parameters of the interaction of Anabaena NTRC with 2-Cys Prx from different sources.

Reactions were performed at a fixed concentration of AnabNTRC (2 μm) and variable concentrations of the 2-Cys Prxs in the presence of 0.25 mm NADPH. Data are means ± sd of three determinations.

Source Vmax Km Kcat Kcat/Km
μmol min−1 μm s−1 μm−1 s−1l
Anabaena 2-Cys Prx 169 ± 6.4 2.84 ± 0.3 2.81 ± 0.1 0.99
Rice 2-Cys Prx 170.1 ± 6.98 7.9 ± 0.4 1.42 ± 0.06 0.18
Arabidopsis 2-Cys Prx A 138 ± 2.9 5.9 ± 0.4 1.15 ± 0.1 0.19
Arabidopsis 2-Cys Prx B 114.3 ± 0.7 6.3 ± 0.2 0.95 ± 0.007 0.15