Table I. Kinetic parameters of the interaction of Anabaena NTRC with 2-Cys Prx from different sources.
Reactions were performed at a fixed concentration of AnabNTRC (2 μm) and variable concentrations of the 2-Cys Prxs in the presence of 0.25 mm NADPH. Data are means ± sd of three determinations.
Source | Vmax | Km | Kcat | Kcat/Km |
μmol min−1 | μm | s−1 | μm−1 s−1l | |
Anabaena 2-Cys Prx | 169 ± 6.4 | 2.84 ± 0.3 | 2.81 ± 0.1 | 0.99 |
Rice 2-Cys Prx | 170.1 ± 6.98 | 7.9 ± 0.4 | 1.42 ± 0.06 | 0.18 |
Arabidopsis 2-Cys Prx A | 138 ± 2.9 | 5.9 ± 0.4 | 1.15 ± 0.1 | 0.19 |
Arabidopsis 2-Cys Prx B | 114.3 ± 0.7 | 6.3 ± 0.2 | 0.95 ± 0.007 | 0.15 |