Figure 4.
Analysis of the lhca5 lhca6 pgr5 triple mutant. A, Visible phenotype of the triple mutant. Plants were grown in a growth chamber (50 μmol photons m−2 s−1, 16-h photoperiod, 23°C) for 4 weeks after germination. B, High-chlorophyll-fluorescence phenotype of the lhca5 lhca6 pgr5 triple mutant. Dark-adapted seedlings of the indicated plants were illuminated at 100 μmol photons m−2 s−1 for 1 min, and a chlorophyll fluorescence image was captured by FluorCAM 700MF. C, Measurement of ΦPSII. ΦPSII was calculated as (Fm′ – Fs)/Fm′. Values are means ± sd (n = 5). D, Analysis of the thylakoid proteins. Thylakoid membrane proteins from the indicated plants were separated by SDS-PAGE and immunodetected with specific antibodies against NdhL, NDF1, FKBP16-2, NDH18, PsaA, D1, and Cyt f. Thylakoid proteins were loaded on an equal chlorophyll basis. E, Semiquantitative analysis of thylakoid proteins as in Figure 2B. The protein levels in the wild-type (WT) plants were defined as 100%. Values are means ± sd (n = 3).