Figure 5.

Analysis of thylakoid proteins from wild-type (WT), lhca5, lhca6, and lhca5 lhca6 plants under high-light conditions. A, Four-week-old plants cultured at 50 μmol photons m⁻² s⁻¹ were exposed to high light (500 μmol photons m⁻² s⁻¹) for 2 d (16-h photoperiod) in a temperature-controlled chamber at 23°C and then harvested after a 10-h illumination on the last day. Freshly isolated thylakoid proteins were separated by SDS-PAGE and then immunodetected with the specific antibodies indicated. Thylakoid proteins were loaded on an equal chlorophyll basis. B, Semiquantitative analysis of thylakoid proteins as in Figure 2B. The protein levels in the wild type grown at 50 μmol photons m⁻² s⁻¹ were defined as 100%. Values are means ± sd of three independent experiments using different plant materials. C, Thylakoid protein complexes isolated from high-light-treated plants were separated by BN-PAGE and then subjected to 2D SDS-PAGE. The proteins were immunodetected with the specific antibodies indicated. Arrows indicate the intact NDH-PSI supercomplex (I), the smaller NDH-PSI supercomplexes detected in lhca5 and lhca6 (II), monomeric NDH detected in lhca5 lhca6 (III), and the putative NDH-PSI subcomplex containing NDH18 and NDF1 detected in lhca5 (*).