Figure 2.

Pro metabolic changes associated with ProDH induction in the HR. A, Quantification of ProDH1, ProDH2, P5CDH, and P5CR transcripts levels in leaves isolated at 0, 6, 8, and 12 hpi with the avirulent bacterium Pst-AvrRpm1. Pathogen infiltration and transcript quantification were performed as described in Figure 1. One representative of three independent infection experiments (each one including at least three plants and four leaves per time point) is shown. B, Free Pro and P5C content (μg g⁻¹fresh weight [FW]) was determined in aliquots of the leaf samples described in A. Data represent means ± sd of Pro values in five independent experiments (three leaves from three plants each). Statistically significant differences (P < 0.01, ANOVA) are shown using different lowercase letters.