Figure 3.
SA stimulates the expression of ProDH1 in HR. A and B, Effects of exogenous SA on ProDH mRNA and protein levels in wild-type plants. Leaves infiltrated with the indicated SA concentrations were excised at 3 h post treatment to monitor the abundance of ProDH1 and ProDH2 transcripts (A) as well as ProDH protein (B). C, Requirement of SID2 and NPR1 for ProDH1 activation by Pst-AvrRpm1. Wild-type (wt), sid2-2, and npr1-1 leaves were infiltrated with Pst-AvrRpm1 and excised at 6 and 24 hpi to determine the abundance of ProDH1 transcript in all three plants. Pathogen infiltration and transcript and protein quantification were performed as described in Figure 1. One representative from three (C) or four (A and B) independent experiments is shown for each assay. Each experiment included at least three plants and at least three leaves isolated from different plants per time point.