Figure 6.

Distribution and appearance of peripheral and central chloroplasts within individual chlorenchyma cells. Protoplasts were prepared as described in “Materials and Methods.” Diluted aliquots of the preparation were spotted onto the microscope slide, and a coverslip was gently applied. The pressure from the coverslip disrupted the protoplasts so that the individual chloroplasts came to rest in the same focal plane and the CC became dispersed, so that individual chloroplasts become visible. A, Example of a micrograph used to quantify the numbers of P-CP and C-CP within an individual cell. Chloroplasts were counted from micrographs, and individual P-CP and C-CP were marked with triangles and circles, respectively, to avoid redundant counting. Numbers for this example are shown. Results are summarized in Table II. B, Magnification of a dispersed CC. Individual C-CP are visible and can be distinguished from P-CP by their size and shape. C, Magnification of P-CP. P-CP are larger than C-CP and have a distinguishing doughnut-like shape. Magnification in A, 100×; in B and C, 400×.