Table I. Substrate-dependent photosynthetic oxygen evolution in B. sinuspersici cells and subcellular compartments.
Different substrates (HCO3−PGA, OAA, Pyr, and pBQ) were tested for their ability to induce oxygen evolution in the two different chloroplasts types, the CC, and isolated TC. Control experiments without light and with the C3-cycle inhibitor GA are shown as well. Values are averages of the maximum rates of four independent experiments and are expressed as μmol oxygen mg−1 chlorophyll h−1. Numbers in parentheses represent se. Oxygen evolution rates less than 1 μmol oxygen mg−1 chlorophyll h−1 were considered to be insignificant. The last column shows intactness (%) measured by ferricyanide (FC)-dependent photosynthetic oxygen evolution determined from isolated intact and osmotically shocked chloroplasts. n.d., Not determined.
| Substrate | +HCO3− | −HCO3− | −Light | +GA | +3-PGA | +OAA | +Pyr | +OAA,+Pyr | pBQ | FC |
| TC | 83 (7.2) | 24.9 (2.0) | <1 | n.d. | <1 | n.d. | n.d. | n.d. | 95 (4.1) | n.d. |
| CC | 42 (3.1) | <1 | <1 | <1 | 53 (4.3) | n.d. | n.d. | n.d. | 90 (11.5) | 74 (4.9) |
| C-CP | 40 (1.8) | <1 | <1 | n.d. | 82 (11.0) | n.d. | n.d. | n.d. | n.d. | 77 (7.8) |
| P-CP | 1 (0.4) | <1 | <1 | n.d. | 69 (3.4) | <1 | <1 | <1 | 111 (16.1) | 86 (9.2) |