Figure 1.
A, Schematic presentation of the Synechocystis FeCH (coded by the hemH gene) with catalytic and C-terminal CAB domains connected by region II. WT, Wild type. B, Strains with truncated FeCH used in this study; WTzeo and ΔH324 were already described (Sobotka et al., 2008b). The ΔhemH deletion was combined with the FLAG-hemH background (see below) to construct the FLAG-hemH/ΔhemH strain, which expressed the tagged version as the only FeCH enzyme in the cell. C, Constructs used to express full-length and truncated versions of FeCH fused to a protein tag in Synechocystis. 3xFLAG-tagged FeCHs were expressed under the control of the Synechocystis petJ promoter; the His-C-tn strain expressed the 63-residue-long C-terminal fragment of Synechocystis FeCH as a small His6-tagged protein under the control of the Synechocystis psbAII promoter.