Figure 5.
Promoter-GUS expression in transgenic soybean hairy root lines of resistant NIL-R infected with PA3 SCN. Promoter-GUS constructs representing 10 genes up-regulated in NIL-R identified from the microarray analysis and the nematode-inducible AtWRKY23 were infected with SCN and stained for GUS expression at 5 dpi. A, AtWRKY23 (At2g47260). B, Glyma15g04570.1. C, Glyma15g06130.1. D, Glyma14g06080.1. E, Glyma09g34110.1. F, Glyma01g42500.2. G, Glyma01g42440.1. H, Glyma18g43750.1. I, Glyma18g18060.1. J, Glyma19g04410.1. K, Glyma13g35100.1. Images are representative of at least five independent hairy root lines for each promoter-GUS fusion construct. N, Nematode; Syn, syncytia. Bars = 500 μm.