Figure 3.

PHB production in leaf samples of greenhouse-grown transplastomic lines over three regeneration cycles. Regeneration cycles at right are as follows: R1, lines obtained after plastid transformation of pCAB and isolation of regenerant; R2, R1 lines that were subjected to one additional cycle of shoot regeneration from an excised leaf; R3, R2 lines that were subjected to another additional cycle of shoot regeneration from an excised leaf. Samples from the R2 regeneration cycle of line 4 and the R1 regeneration cycle of line 10 were not analyzed. Data points represent averages of two mature to senescent leaf samples harvested from the indicated line. One of these samples was harvested from leaf 4 to 7 (where the oldest leaf of a plant is defined as leaf 1), and the other sample was harvested from leaf 8 to 12. Error bars represent sd. dwt, Dry weight.