Table I. Maps of plastid transformation vectors used in this study.

Maps show inserts cloned within bacterial vector pUC19 (Yanisch-Perron et al., 1985). Abbreviations and notes are as follows: left flank of plasmids pUCaadA, pCA, and pCAB contains the complete coding sequence of psbA (D1 protein of PSII), left flank DNA is homologous to the reverse complement of nucleotides 536 to 1,597 of the tobacco plastome (Herz et al., 2005); aadA, gene encoding aminoglycoside 3′-adenyltransferase from E. coli providing spectinomycin/streptomycin resistance (Svab and Maliga, 1993); right flank of plasmids pUCaadA, pCA, and pCAB contains 3′ UTR of psbA, trnH (tRNA-His), and part of rpl2, right flank DNA is homologous to the reverse complement of nucleotides 155,398 to 155,943 and 1 to 530 of the tobacco plastome (Herz et al., 2005); 5′ UTR T7g10, 5′ UTR of gene 10 of bacteriophage T7 (Kuroda and Maliga, 2001), DNA homologous to nucleotides 22,904 to 22,969 of the bacteriophage T7 genome (EMBL accession no. V01146; Dunn and Studier, 1983); phaC_As, gene encoding PHB synthase from Acinetobacter sp., homologous to nucleotides 2,351 to 4,123 of the Acinetobacter sp. PHA biosynthetic gene locus (EMBL accession no. L37761; Schembri et al., 1995); rps19/rpl22 spacer, DNA homologous to nucleotides 86,353 to 86,399 of the tobacco plastome, contains an intergenic region between rps19 and rpl22 (Herz et al., 2005); phaA_As, gene encoding thiolase from Acinetobacter sp., homologous to nucleotides 4,206 to 5,384 of the Acinetobacter sp. PHA biosynthetic gene locus; 5′ UTR rbcL, 15 nucleotides of the 5′ leader sequence of the gene encoding the large subunit of Rubisco, homologous to nucleotides 57,580 to 57,594 of the tobacco plastome (Svab and Maliga, 1993); psbD/C spacer, DNA homologous to nucleotides 35,463 to 35,517 of the tobacco plastome, contains the sequence region upstream of the gene encoding PSII 44-kD protein (psbC), which is overlapped by the coding region of the psbD gene encoding the PSII D2 protein (Herz et al., 2005); phaB_Bm, gene encoding acetoacetyl-CoA reductase from B. megaterium, homologous to nucleotides 4,758 to 5,501 of the B. megaterium PHA gene cluster (EMBL accession no. AF109909; McCool and Cannon, 1999).