Table I. Molecular and biochemical evidence for nucleus-encoded algal genes in E. chlorotica.
Protein | Method | Gene | Source |
Fucoxanthin a/c chlorophyll-binding protein | 35S immunolabeling | fcp | Pierce et al. (1996, 2003) |
Fucoxanthin a/c chlorophyll-binding protein | PCR | fcp | Pierce et al. (2007) |
Light-harvesting complex-binding protein 1 | 35S immunolabeling | lhcv-1 | Hanten and Pierce (2001) |
Light-harvesting complex-binding protein 1 | PCR | lhcv-1 | Pierce et al. (2007) |
Light-harvesting complex-binding protein 2 | PCR | lhcv-2 | Pierce et al. (2007) |
Light-harvesting complex-binding protein 3 | PCR | lhcv-3 | Pierce et al. (2009) |
Light-harvesting complex-binding protein 4 | PCR | lhcv-4 | Pierce et al. (2009) |
Oxygen evolution complex protein | PCR | psbO | Rumpho et al. (2008) |
Oxygen evolution complex protein | Quantitative PCR | psbO | K. Soule and M.E. Rumpho (unpublished data) |
Phosphoribulokinase | 35S immunolabeling | prk | Rumpho et al. (2009) |
Phosphoribulokinase | PCR | prk | Rumpho et al. (2009) |
Phosphoribulokinase | Quantitative PCR | prk | Soule (2009) |
Uroporphyrinogen decarboxylase | PCR | uroD | Pierce et al. (2009)a |
Magnesium chelatase subunit D | PCR | chlD | Pierce et al. (2009)a |
Magnesium chelatase subunit H | PCR | chlH | Pierce et al. (2009)a |
Chlorophyll synthase | PCR | chlG | Pierce et al. (2009)a |
Chlorophyll synthesis | 14C labeling | NDb | Pierce et al. (2009) |
Where identical data were presented in more than one publication, the earliest publication date is noted.
ND, Not determined.