FIGURE 2.

XIAP regulates β-actin polymerization and cytoskeleton formation. A and B, the soluble and insoluble fractions were isolated, and G-actin, F-actin, and GAPDH were detected by Western blotting. C, the induction of F-actin polymerization upon treatment with EGF (50 ng/ml) was evaluated by spectrophotometry in WT, XIAP^−/−, and XIAP^−/−(HA-XIAP) cells (p < 0.05). D, the indicated cells were stained with Oregon Green 488-conjugated phalloidin, and cytoskeleton and F-actin fibers were observed by confocal microscopy. E, the induction of F-actin polymerization upon treatment with EGF (50 ng/ml) was evaluated by flow cytometry in the various cell lines indicated.