FIGURE 1.

Sup35 proteins used. A, sequence alignment of Sup35 prion domains from S. cerevisiae (cer), S. mikatae (mik), S. kudriavzevii (kud), S. paradoxus (par), and S. bayanus (bay). Only differing amino acid residues are shown. Gaps are shown by highlighted dashes. B, scheme of the hybrid Sup35 proteins. The Sup35 N-terminal domain (N) was replaced with analogous domains from the indicated species. In one set of constructs, the 3-HA tag was placed after residue 251. M, middle domain; C, C-terminal domain.