FIGURE 4.

Electrophoretic analysis of co-polymerization of hybrid Sup35 proteins. Yeast cells with the indicated [PSI⁺] variants were transformed with centromeric plasmids producing hybrid Sup35-3-HA proteins. Cell lysates were loaded onto gels without boiling and run for half a distance. The whole gels were then boiled, and the electrophoretic separation was continued. The gels were blotted, and the blots were stained with antibody to the Sup35-cer N-terminal and middle domains. Sx-3HA, hybrid Sup35-3-HA proteins; Sc, Sup35-cer lacking the tag. For ease of comparison, the values for [PSI⁺] loss and transmission are given below the gels. The W1 panel also shows lysates of the cells having lost the plasmid encoding Sup35-cer (asterisk).