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. 2011 Feb 28;286(18):15875–15882. doi: 10.1074/jbc.M111.227819

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Ca_vβ-expression. A and B, Ca_Vβ1(β1), Ca_Vβ2(β2), Ca_Vβ3(β3), and Ca_Vβ4(β4) in brain (A) and expression of Ca_vβ2 in heart (B) (100 μg/lane). C, Ca_Vβ2 transcript expression in cardiac poly(A⁺)-RNA (10 μg/lane) from WT (lane 1), Ca_Vβ2^−/flox/MerCreMer^tg/0 (mock, lane 2), Tamoxifen-treated Ca_Vβ2^−/flox/MerCreMer^tg/0 (KO, lane 3), Ca_Vβ2^+/flox/MerCreMer^tg/0 (lane 4), and Ca_Vβ2^+/flox/MerCreMer^0/0 (lane 5). Loading controls are as follows: GAPDH expression (middle) and ethidium bromide stain (bottom). D, Ca_Vβ2 protein expression in heart (45 μg) from WT (lane 1), Ca_Vβ2^+/− (lane 2), Ca_Vβ2^−/flox/MerCreMer^tg/0 (mock, lane 3), and Tamoxifen-treated Ca_Vβ2^−/flox/MerCreMer^tg/0 (KO) (lane 4). Lane 5, 40 μg; lane 6, 80 μg. Protein expression in brain is shown as control (exposed for 15 min when compared with 1 min (lanes 1–4)). GAPDH was used as a loading control. E, estimation of the Ca_Vβ2 protein expression levels in hearts from controls (lanes 1, 3, and 5) and KO (lanes 2, 4, and 6) using the Ca_Vβ2 antibodies 425 (upper) and 424 (lower). Coomassie Brilliant Blue stain was used as a loading control.