FIGURE 11.

Inhibition of CerS but not de novo synthesis inhibits plasma membrane rupture that is not specific to UV-C irradiation or MCF-7 cells. A, MCF-7 cells were preincubated with myriocin (Myr; 100 nm), fumonisin B1 (50 μm), or vehicle (Veh; 0.1% methanol) for 30 min and then treated (UV) or not treated (NT) with 10 mJ/cm² UV-C. The cells were then incubated for 24 h, and LDH release was assessed. Data represent mean and error bars represent S.E. of three independent experiments. B, MCF-7 cells were preincubated with myriocin (100 nm), fumonisin B1 (50 μm), or vehicle (0.1% methanol) for 30 min and then treated with 3 nm TNF-α or PBS for 48 h after which LDH release was assessed. Data represent mean and error bars represent S.E. of three independent experiments. C, HeLa cells were preincubated with myriocin (100 nm), fumonisin B1 (50 μm), or vehicle (0.1% methanol) for 30 min and then treated (UV) or not treated (NT) with 10 mJ/cm² UV-C. The cells were then incubated for 24 h, and LDH release was assessed. Data are mean and error bars represent S.E. for three independent experiments. A–C, statistical significance was determined by two-way ANOVA and Bonferroni post hoc tests comparing UV-C/inhibitor-treated with UV-C/vehicle-treated samples. *, p < 0.05; ***, p < 0.001.