FIGURE 2.

UV-C induces accumulation of multiple sphingolipid species. MCF-7 cells were treated (UV) or not treated (NT) with 10 mJ/cm² UV-C followed by incubation for the indicated durations, and cells were harvested for analysis by HPLC/MS. Total Cer (A), dHCer (B), SM (C), and HexCer (Hex) and lactosylceramide (Lac) (D) are shown. Data represent mean and error bars represent standard error of three independent experiments, and sphingolipid levels were normalized to total lipid phosphate. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus untreated control cells.