FIGURE 5.

Bcl-xL overexpression inhibits late UV-C-induced cell death and late Cer generation. MCF-7 cells stably expressing Bcl-xL or control vector (Vec) were treated with 10 mJ/cm² UV-C followed by incubation for the indicated durations. Cells were then harvested for further analysis. A, cell death was determined by trypan blue exclusion. B, effect of Bcl-xL overexpression on UV-C-induced changes in total Cer. Data represent mean and error bars represent S.E. of three independent experiments, and Cer levels were normalized to total lipid phosphate. Statistical significance was determined by two-way ANOVA and Bonferroni post hoc tests. ***, p < 0.001 versus time-matched MCF-7/Vec cells.