FIGURE 8.

Inhibition of Cer synthases but not de novo synthesis inhibits programmed cell death as determined by trypan blue exclusion. MCF-7 cells were preincubated with myriocin (Myr; 100 nm), fumonisin B1 (50 μm), or vehicle (Veh; 0.1% methanol) for 30 min and then treated (UV) or not treated (NT) with 10 mJ/cm² UV-C. The cells were then incubated for the indicated durations. Cell death was determined by trypan blue exclusion. Data represent mean and S.E. of three independent experiments. ***, p < 0.001 versus time-matched UV-C/vehicle-treated cells.