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. 2011 Mar 14;286(18):16030–16038. doi: 10.1074/jbc.M110.199398

FIGURE 4.

FIGURE 4.

Activation of AMPK inhibits MMP-9 expression from MEFs. A, WT and AMPKα−/− MEFs were treated with 0.25 and 0.5 mmol/liter AICAR. The phosphorylation of AMPKα (p-AMPKα) and ACC (p-ACC) after AICAR treatment was examined by Western blotting. B, after 12-h AICAR treatment, the MMP-9 concentrations in cell culture medium of WT and AMPKα−/− MEFs were measured by ELISA. C, WT and AMPKα−/− MEFs were treated with 25 and 50 μmol/liter A769662. The phosphorylation of AMPKα and ACC after A769662 treatment was examined by Western blotting. D, after 12-h A769662 treatment, the MMP-9 concentrations in cell culture medium of WT and AMPKα−/− MEFs were measured by ELISA. E, WT MEFs were transduced with adenovirus vectors expressing GFP (Ad-GFP) or the DN form of AMPKα (Ad-DN). Whole cell lysates of transduced MEFs were examined by Western blotting to confirm gene transduction. F, MMP-9 concentrations in cell culture medium of transduced WT MEFs were measured by ELISA. A, C, and E, representative blots are shown. *, p < 0.05; **, p < 0.01; NS, not significant. Error bars, S.E.