FIGURE 3.

Effect of βME and ethanol on L1 adhesion in cells expressing WT-L1 and mutant-L1. A, βME inhibition of L1 adhesion in WT-L1 (white bars), E33C/Y418C-L1 (black bars), E33C-L1 (gray bar), or Y418C-L1 (gray bar). B, reversibility of βME inhibition on E33C/Y418C-L1 adhesion. Cells expressing WT-L1 (white bars) or E33C/Y418C-L1 (black bars) were pretreated with βME for 15 min, followed by washing with PBS supplemented with 30 mm βME (βME; left bars) or with plain PBS (PBS; right bars) to remove the βME. Assays were conducted using the same solutions as the wash. C, inhibition of E33C/Y418C-L1 adhesion by 100 mm ethanol, 30 mm βME, or both. Data are mean ± S.E. from 4–9 determinations. *, p < 0.01; ***, p < 0.0001.