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preSTIM2 and the STIM2 SPF are localized in the cytosol. HEK293T cells transfected with STIM2FLAG, STIM2L89H, SPF91, or STIM2-FcFLAG were co-stained with anti-FLAG (green) to visualize FLAG-tagged proteins and anti-calnexin (red) to visualize the ER and imaged by confocal microscopy. All images are from the middle of the cell. Scale bar, 10 μm.
