FIGURE 6.

preSTIM2 and the STIM2 SPF regulate gene transcription. A and B, shown are basal- and PMA-induced NFAT-SEAP (A) or NF-κB-SEAP (B) reporter gene activity in HEK293T cells transiently transfected with indicated constructs. Data represent the mean ± S.D. for n = 3 individual wells and are representative of four (NFAT) and five (NF-κB) experiments. WT, wild type. ns, not significant; *, p < 0.05; **, p < 0.01. C, preSTIM2 and the SPF specifically interact with Ca²⁺/calmodulin. Slight degradation of preSTIM2 is visible in this experiment. Identical results were obtained in two individual experiments. IP, immunoprecipitation; IB, immunoblot.