FIGURE 7.

Markers for epithelial-to-mesenchymal transitions are regulated upon ectopic expression of siRNA-resistant mutant p53 regardless of stable knockdown of endogenous wild-type p53 in MCF-10A cells. A, C, and E, Western blots were prepared with extracts from parental MCF-10A cells (lane 1), p53-KD MCF-10A cells (lane 2), MCF-10A cells in which a mutant p53 was ectopically expressed (lanes 3, 5, 7, and 9) and MCF-10A cells in which a mutant p53 was ectopically expressed along with knockdown of endogenous wild-type p53 (lanes 4, 6, and 8). The blots were probed with antibodies against E-cadherin (A), ZO-1 (A), p53 (A, C, and E), laminin V (C), β-catenin (C), Snail (E), Slug (E), Twist (E), and actin (A, C, and E). B, the experiments were performed as in A except that two MCF10A cell lines in which mutant G245S or R273H was ectopically expressed along with knockdown of endogenous wild-type p53 were used. D, the experiments were performed as in C except that two MCF10A cell lines in which mutant G245S or R273H was ectopically expressed along with knockdown of endogenous wild-type p53 were used. F, the experiment was performed as in A except that the blots were probed with antibodies against p53, p21, Bax, and Puma, respectively.